Ethnic Groups and Conflicts

The conflict between whites and blacks is not devoid of social perception, conformity, and social recognition as crucial components. This conflict has been witnessed in countries that have citizens with both white and black origins. Advertising We will write a custom essay sample on Ethnic Groups and Conflicts specifically for you for only $16.05 $11/page Learn More In many countries, such as the United States of America and South Africa, the conflict between the two ethnic groups has been bad, and in some cases has turned out to be ugly with members engaging violently. The conflicts between members of different ethnic groups have been attributed to social class, politics, and old stereotypes as practiced by most people. The conflicts, sometimes, arise when a group that feels discriminated against in a given political or social field tries to strengthen its position. In the African countries for example, the conflict between whites and blacks are mainly attributed to the fact that whites are known to have colonized most of the nations in the continent. The process of colonization is known to have been characterized by cruel treatment of the African slaves (Turnbull, 1965). On the other hand, the conflicts in the two American continents originate from the fact that the whites are historically known to have been the slave masters of the blacks. As slave masters, the whites subjected blacks to miserable living conditions. The fact that this happened a long time ago is not a reason strong enough to heal the wounds to enable members of the ethnic communities to live in peace. There are people in one group who look down upon those in the other group. It is also important to note here that there are those who have developed a feeling of inferiority. This has, in the end, resulted in conflicts that have prompted them to acquire a given level of social status (Shiraev Levy, 2010). Conformity in Blacks and Whites Conformity refers to a s tate of life in which people are compelled by circumstances around them to change their attitudes, and their general behaviour. This is usually done with the aim of holding onto a given social or group norm. According to Shiaev and Levy (2010), it is a natural human conformity approach for people to develop the need to maintain positive relationships with those around them. This has a final target of ensuring that the majority are accommodated. Under conformity, it is known that people tend to surmount the obstacles that exist, and which block them from living according to the expectations of the society. Advertising Looking for essay on ethnicity studies? Let's see if we can help you! Get your first paper with 15% OFF Learn More This enables people to live in good graces with other members of the society who are of different ethnic origin. Judgment is usually employed when there is a feeling that failure to change one’s natural behavior can result in a non-posi tive consequence by the members of the other ethnic community. This explains why, for example, whites tend to practice lifestyles common among the blacks whenever they visit African countries. This involves, among many other aspects, the way their dressing is done. It has also been witnessed in American politics that certain blacks have rallied behind certain white candidates for elective posts to avoid situations that would be interpreted in ethnic terms. When people embrace such behavior, there is a general reduction in the tension that would otherwise build up between the members of the two different ethnic groups (Verkuyten, 2005). Relationship between Social Perception and Social Cognition It can be stated here that social perception is an element that falls under social cognition. Through this element, an individual finds it possible to perceive the manner in which people from other ethnic groups carry out their duties within a given social world. Social cognition refers to â€Å"the way people interpret, remember, and process information about the world and themselves† (Shiraev Levy, 2010, p. 256). Through social perception, it is possible for one to understand how the natural world goes together with socialization, and how each one of them affects the other. On the other hand, social cognition refers to the manner in which the perceived information is gathered, stored, and processed. In order to explain the concept of perception, and enhance a further understanding of cognition, contact hypothesis was formulated. This holds that the negative attitudes that exist between members of any two conflicting groups can be mediated and addressed through contact of the parties in the conflict. In the conflict between blacks and whites, the greatest social perceptions that fuel the conflict between the two ethnic groups on a daily basis are economy, social status, and politics (Verkuyten, 2005). It is generally believed that politics is responsible fo r the many economic challenges blacks face, and this is what has fueled the conflicts between the two groups.Advertising We will write a custom essay sample on Ethnic Groups and Conflicts specifically for you for only $16.05 $11/page Learn More Addressing the Social Perceptions The application of the contact hypothesis as a valid approach in solving the conflicts between blacks and whites has been barred by some of the social perceptions embraced by the conflicting groups. It is important for governments all over the world to address the problem of economic disparity among members of the two communities, especially the black community. This could be achieved through the government ensuring that all people get access to equal economic opportunities regardless of their race or ethnic origin (Verkuyten, 2005). It is also important for the political system in countries where such conflicts exist to embrace a unifying approach while doing politics. In the U nited States for example, the greatest part of the conflict was fueled by the perception that the whites had put in place political measures that would ensure their supremacy over the blacks. This was sometimes evident when measures suggested to enhance equality among the two groups were opposed with the greatest force (Shiraev Levy, 2010). Finally, it is important for the class system to be addressed to ensure that there is successful integration of all cultures among the members of the two groups. This can be addressed when children from both communities are encouraged to school together. Through this approach the children grow up with a clear understanding that all people are equal regardless of their race or ethnic origin (Verkuyten, 2005). Conclusion For many decades, there has been discord between blacks and whites all over the world. Social perceptions started and propelled by politics have resulted in the hatred that characterizes the relationship between blacks and whites. Social cognition and conformity have been identified as some of the greatest forces that prevent the integration and coming together of the two groups. Much has been achieved in efforts to reduce the conflict between the two ethnic groups that have, in some instances, engaged in very violent confrontations (Horowitz, 2001). However, it will take several decades for the two communities to engage in full cooperation, integration and trust. References Horowitz, D. L. (2001). The Deadly Ethnic Riot. Berkeley: University of California Press.Advertising Looking for essay on ethnicity studies? Let's see if we can help you! Get your first paper with 15% OFF Learn More Shiraev, E. B., Levy, D. A. (2010). Cross-cultural psychology: Critical thinking and contemporary applications. Boston: Pearson/Allyn Bacon. Turnbull, C. M. (1965). Wayward Servants: The Two Worlds of the African Pygmies. Garden City, New York: The Natural History Press. Verkuyten, M. (2005). The Social Psychology of Ethnic Identity: European Monographs in Social Psychology. New York: Psychology Press. This essay on Ethnic Groups and Conflicts was written and submitted by user Raiden Keller to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Contrast First Draft essays

Comparison/Contrast First Draft essays An aggressive retort by the United States will have a better outcome than no action at all as a result of the terrorists acts in New York. If you live in America right now you cant turn around without being assaulted by the media blitz on the recent attacks of the World Trade Centers. Until this incident we had considered ourselves immune to any foreign massive attack on our own soil. How wrong that turned out to be! Even with all of our technologies in the military we had no way to stop these people. I watch CNN and listen to all of the different opinions that people have given, and frankly am aghast at what I hear. When people get on television and give interviews I first look at their credentials, which are most often supplied. When I see PhDs. MDs and other impressive educational achievements following their name I automatically give them some consideration. What do some of these folks have to say? More than a few of these people dont think that retaliation by us is the right thing to do. They dont want to beget violence with the same. If you think of Jesus and his philosophy of turning the other cheek, you have to agree.... somewhat. Why should America show the rest of the world how petty we are by making such a big thing out of a few thousand people getting killed by some lunatics? Dont forget also these suspects are renegades, not claimed by any country. This makes for a very elusive target, in that we shouldnt bomb a whole country for the mistakes of a few. In watching the coverage, I was struck by the tone of passive acquiescence. Not once, in hours of listening, did I hear anyone express righteous anger. No one said, coldly, but with deadly seriousness, People responsible for this are going to die. Our military leaders speak of how we cant act rashly and the need for caution, which most likely means nothing will be do ...

Ehtnographic Study paper Essay Example | Topics and Well Written Essays - 1500 words

Ehtnographic Study paper - Essay Example Regarding the students in the entire school, these students’ ethnic background(s), language(s), ages, educational level of community members, behavioral norms and values, and socioeconomic statuses will be evaluated and thoroughly analyzed. Here we will analyze primarily the students in the entire school, with a philosophy of diversity centering upon the particular class. II. Background The ethnic mix of the learners, both in the class and in the school, is diverse. The school’s student body is predominantly African-American, although there are small amounts of other races present in the student body. The school used to be more racially diverse years ago. Regarding students’ socioeconomic status, this is diverse as well. The SES of the learners at this high school is varied, but a lot of the students tend to come from backgrounds with socioeconomically disadvantaged status. The primary language spoken is English, and in some cases, Ebonics (although not formally recognized by some as a bona fide language) is widely spoken by the student body. The type of community surrounding the school is in the city. Therefore, this school is an urban school. This school has special populations (special education, ESL/ELL, gifted). This school has a comprehensive special education program. Moreover, however, it has a large gifted population in its 7th-12th grade program. A certain percentage of the 7th and 8th graders in the Gifted program remain to go on for high school there, so there is that factor to consider, too. It is a keystone of any teacher’s philosophy of diversity that the classroom be a place of inclusiveness—whether students be Black, white, Latino, Asian, Native American, or of mixed race(s) or races not named here, and be of whatever socioeconomic status, religion, national origin, gender, sexual orientation, disability, or cultural affiliation—that students feel that they are equals in the classroom regardless of the teacher’s cultural lens. An inclusive philosophy dictates that each student should have the same opportunity to learn their subject. This includes being cognizant of striving against favoritism, especially based on gender or race. Students who are from lower levels socioeconomically should be given a fair chance to succeed by providing them with the tools they need to succeed in an environment which has typically favored the dominant culture’s hegemonic social strata. A level playing field is key. Strategies include having class materials available such as extra paper and pens. Teachers should want students of various national origins and religions to feel comfortable enough to express themselves within the dynamic of their own backgrounds. Especially with students who have ESL/ELL/bilinugal/multilingual backgrounds, teachers should want to make sure that their class is accessible to their language capabilities by supporting their learning with extra attention in order to check for understanding so that they don’t fall behind. Additionally, teachers should like to extend their help to students of different genders and sexual orientations who may suffer discrimination from other students—and try to combat that discrimination within the classroom by having an open-door policy. The teacher should wish to facilitate a learning

Leadership in Customer Service Article Example | Topics and Well Written Essays - 750 words

Leadership in Customer Service - Article Example There are so many kinds and interpretations of a leader, but which one does it take to make a customer-service-driven business successful? Leadership needs to have a vision of what is wanted to achieve. Leaders possess not only this but are driven by passion—the strength and the energy to work against the odds to achieve the vision (Williams, 2005). Great leaders delegate and empower. Leaders do not just pin point on people and tell them what to do instead they create structure, allocate responsibility, help create systems, provide support and training and resources, and empower subordinates to make decisions thus making them feel significant in the business group. Through empowerment, a leader is set differently from an authoritarian. A leader does not dictate but respect employees and treats these people as customers as well. Great leaders sometimes have to take tough decisions but there's always respect for the subordinates (Williams, 2005). Workers are encouraged to: participate in the action plan; develop awareness of the goals; participate in the change plan; have reasonable expectations and attitude. Communication flow is always open to a leader and is never one way. Successful business leaders are always keeping their people informed thus key performance indicators are displayed; targets are set, accomplished and celebrated. Everyone is made to feel as part of a community to create a sense of unity (Williams, 2005). A leader motivates people to do what is important and leads an example. There's a famous story about a group of visitors to Disney who was walking in the Magic Kingdom when they saw a grey-haired man walk out of his way to pick up a piece of litter. One of the members of the group approached the man and asked, "How many custodians are there here?" The man replied, "45,000". The guest was surprised at so many.

Effective marketing planning requires full research of the marketing Essay

Effective marketing planning requires full research of the marketing environment. Discuss what needs to be considered when under - Essay Example An effective marketing plan requires a research on the drivers of demand in the business environment. This may entail secondary research or primary research, or both in order to investigate consumer behavior and motivation. Some of the aspects contained in an effective marketing plan are demographic and social changes that may affect sales of the company’s product, for instance a change in the lifestyles and the age structure. New technologies that may open up some markets while at the same time closing some. Economic trends that have an impact on the consumer purchase decision, as well as political environment that alters public spending due to aspects like taxation. By conducting a marketing audit, a firm is able to explore its internal capabilities (Alison, 2011, p 23). Effective marketing planning also calls for an examination of the external environment to a firm, which entails focusing on the existing marketing strategies; the company’s marketing mix, and market s egmentation and segmentation, as well. Scanning and interpreting all these areas of market changes calls for knowledge and experience, and might be the source of competitive advantage. Much of the marketing planning also varies on anticipating the response of competitors. The reason is that interpreting and responding to market changes ahead of t6he competitors may result in high gains of both sales and profits. Marketing audit Marketing audit is a fundamental element of the marketing planning process, which ensures that a firm is profitable. Marketing audit considers both internal and internal influential factors. Most tools for conducting a marketing audit include the SWOT, PESTEL and the porter’s five forces (Bensoussan & Fleisher, 2008, p 23). PESTEL analysis PESTEL analysis is an examination of forces outside the business, but has an impact on firms sales and marketing. These are the political, economic, social, technological and legal aspects. The political element deal s with the impacts of government policy. Government legislation also incorporates legal elements that include taxation policies and laws that govern business operations. Government regulation has the effects on privatization and deregulation policy, which have an overall effect of opening up markets to competition. Companies should not be something for companies to fear, as regulations like lowering pollution may act to spur competitive firms to innovate and lower costs to counter increasing costs of regulation. Economic factors like disposable incomes, unemployment, prevailing interest rates, recession and booms have an impact of a company’s sales. Marketers have to scan and monitor the general economic environment before making any marketing move, as this may have impacts on sales and eventual profitability in a certain time. Social-cultural factors refer to consumers’ patterns of behavior, lifestyles and preferences that affect purchase and buying behaviors (Debra, 2005, p 23). Marketers need to be ware of changes in consumer behavior as a result of change in preferences. Trending social elements affects the demand of a company’s products. Additionally, it is vital to examine the technological changes that happen in the industry, especially changes in information technology, research and development and rate of technology change. The reason is that technology changes affect

Dextranase Enzyme Production

Dextranase Enzyme Production Dextranase definition and its uses Dextran is a collective name given to a large class of homopolysaccharides composed of D-glucans with contiguous a-1, 6 glycosidic linkages (95%), with minor secondary linkages such as a-1, 2, a-1, 3 and a-1, 4 [74]. It is produced by microorganisms such as Leuconostoc mesenteroides, Streptococcus sp., Acetobacter capsulatus and Acetobacter viscus [44]. Dextrans are well soluble in water, have low toxicity, and relative inertness. These properties make dextrans effective water-soluble carriers for dyes, indicators, and reactive groups in a wide variety of applications. They are widely used in the pharmaceutical and biochemical fields. Dextrans of low molecular weight are used as an alternative to blood plasma. They are also used for clinical purposes such as drug delivery [82], and by cross-linking for the production of the chromatographic matrix Sephadex. They are also widely used as both anterograde and retrograde tracers in neurons [94]. On the other hand microbial synthesis of de xtrans in damaged cane and beets or other products containing sucrose is a serious problem in sugar and food industry. Dextran is also a structural component of dental plaque which causes the development of dental caries [78], [85]. Dextranases are enzymes that cleave the a-1,6 glycosidic linkages of dextran to yield either glucose or isomaltose (exodextranases) or isomalto-oligosaccharides (endodextranases), and are only produced as extracellular enzymes by a small number of bacteria and fungi, including yeasts and perhaps some higher eukaryotes [44]. Enzymes in many groups can be classified as dextranases according to function: dextranhydrolases, glucodextranases, exoisomaltohydrolases, exoisomaltotriohydrases, and branched-dextran exo-1,2-alpha glucosidases. In particular the chemical reaction catalyzed is as follows: (1,4-alpha-D-glucosyl)n + (1,4-alpha-D-glucosyl)m à ¢Ã¢â‚¬  Ã¢â‚¬  (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m + 1 These enzymes belong to the family of glycosyltransferases, specifically the exosyltransferases. The systematic name of this enzyme class is: 1,4-alpha-D-glucan: 1,6-alphaD-glucan 6 alpha-d-glucosyltransferase. Other commonly used names include dextrin 6-glucosyltransferase and dextrin dextranase. Many microorganisms are known to produce dextranase, including filamentous fungi belonging to the genera Penicillium, Aspergillus, Spicaria, Fusarium and Chaetomium, bacteria, e.g. Lactobacillus, Cellvibrio, Flavobacterium etc. The only yeasts reported to produce dextranases are members of the family Lipomycetaceae. Only Lipomyces kononenkoae [104] and Lipomyces starkeyi dextranases have been characterized [47]. Potential commercial uses of dextranases include: The synthesis of potentially valuable oligosaccharides [30] Potential mouthwash ingredients since isomaltose may be of significant importance for the prevention of dental caries [40], [41] Clearance of dextran contamination in cane sugar processing [25] Dual-stimuli-responsive drug release as in biodegradable polymer-structured hydrogels of gelatin and dextran [55]. Hydrogels are used for a wide range of biomaterials applications such as: contact lenses, drug delivery vehicles and tissue adhesives. Dextrans are polymers that mimic biological sugars found on tissue surfaces. The dextran hydrogel system with tunable mechanical and biochemical properties appears promising for applications in cell culture and tissue engineering [58] Drug delivery device suitable for delivering drug to the colon [7], [8]. Brondsted et al. studied the application glutaraldehyde dextran as a capsule material for colon-specific drug delivery. The dextran capsules were challenged with a dextranase solution, simulating the arrival of the drug delivery to the colon, so they broke and the drug was released as a dose pump. The outcome highlights the dextran capsules as promising candidates for providing a colon-specific drug delivery Also in site-specific drug delivery systems with the use of antibodies [69] The improvement of brewing yeast strain for beer industry. Due to the rising demand for low-calorie beverages, including beer, recombinant strains of Saccharomyces cerevisiae have been produced by integrating LSD1 gene of Lipomyces starkeyi [101]. S. cerevisiae lacks the ability to produce extracellular depolymerising enzymes that can efficiently liberate fermentable sugar from abundant, polysaccharide rich substrates [75]. By introducing the gene mentioned above, adding an exogenous enzyme during beer fermentation to achieve starch hydrolysis and oligosaccharide reduction can be avoided Carbohydrase activity produced can also be exploited in sensitive chromogenic bioassays for toxicity: a mycotoxin bioassay using the intracellular ÃŽÂ ²-galactosidase activity of Kluyveromyces marxianus has been developed [20] Classification of dextranase based on amino acid sequence Dextranases are dextran-degrading enzymes that form a diverse group of carbohydrases and transferases. The more recent classification divides dextranases into two classes: endodextranases (a-1,6-glucan-6-glucnohydrolase; also referred to as dextranase) and exodextranases ( glucan-1,6-ÃŽÂ ±-glycosidase; also referred to as dextran glucosidases). The Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUB-MB) provides a system of classification depending on the type of reaction catalyzed and product specificity (Table 1). Contrary to that system, the Carbohydrate Active Enzymes (CAZy) database describes the families on terms of structural and mechanical features of these enzymes; enzymes with different substrate specificities are placed in the same family and those that hydrolyze the same substrate are sometimes placed in different families. In another classification system, Henrissat and Bairoch [33] have divided glucosylhydrolases and glycosylt ransferases into five families on the basis of the similarities in the amino acid sequences (Table 2). Table 1: The IUB-MB classification system EC3.2.1.11 Dextranases EC3.2.1.70 Glucan-1,6-glucosidases EC3.2.1.94 Glucan-1,6-isomaltosidases EC3.2.1.95 Dextran-1,6-isomaltotriosidases EC3.2.1.115 Branched-dextran exo-1,2-glucosidases Table 2: Classification of dextran hydrolysing enzymes, based on amino acid sequences. Dextran-glucosidases Families 13 15 Isomaltodextranase Family 27 Isomaltotriosidase Family 49 Endodextranases Families 49 66 (no sequence similarities between the two families) Aoki and Sakano (1997) came up with 4 families [2]. They isolated and sequenced the isopullunase gene (ipuA) from Aspergillus niger ATCC 9642. The gene shows significant amino acid similarity to the dextranase produced by Penicillium minioluteum (PEMDEX) and Arthrobacter sp. (ARTDEX). Since ASNIPU shows great similarity to PEMDEX and ARTDEX, they can be classified as Family 1. In the same fashion, the researchers compared the amino acid sequences of dextranases and dextran-hydrolising enzymes, including ASNIPU. Lipomyces species and Lipomyces starkeyi Lipomyces starkeyi and Lipomyces kononenkoae belong to the Lipomycetaceae family and are the only yeasts reported to produce dextranases. The first Lipomyces species was identified by Robert Starkeyi in 1946 during a study of nitrogen-fixing bacteria: it was then that he discovered L. starkeyi, a fat-producing, ascosporogenous soil yeast. The family Lipomycetaceae was proposed later, in 1952 by Lodder and Kreger von Rij. Lipomyces species can utilize starch as a sole source of carbon. Both species contain highly efficient amylolytic systems, permitting growth on starch with very high biomass yields [97]. The family Lipomycetaceae is known to utilize certain heterocyclic compounds, such as imidazole, pyrimidine, and pyrazine and their derivatives, as sole nitrogen sources [92]. Information on the genome organization and molecular genetics of this group of yeasts is very limited. The ascosporogenous soil yeast L. starkeyi has been reported to produce commercially useful extracellular dextranase activity [97], [52], [53], and it can utilize a variety of other compounds, like hexoses, pentoses, alcohols and organic acids, as sole sources of carbon and energy [46]. The strains of L. starkeyi currently used are NCYC 1436, IGC 4047, ATCC 12659 and its de-repressed mutant ATCC 20825. L. starkeyi dextranases Commercial use of dextranase began in 1940s, mainly by producing low-molecular-weight clinical dextran. Therefore, industrially practical mixed culture fermentation of L. starkeyi and Leuconostoc mesenteroides was capable of producing controlled-size dextrans in order to satisfy clinical use, in which dextranase produced by L. starkeyi hydrolyzed the high molecular weight dextran produced by L. mesenteroides to a controlled size [46]. The enzyme production system of L. starkeyi needs an inducer. Dextran is its normal inducer but it is a relatively expensive carbon source for large-scale fermentations. Also, L. starkeyi is reported to have slow growth and difficulty of avoiding contamination from other microorganisms during growth. With that in mind D. W. Koenig and D. F. Day (1989) undertook to establish conditions which would minimize the cost of the inducer for producing an enzyme by using a de-repressed mutant of L. starkeyi ATCC 12659 grown on glucose. Thus the mutant ATCC 20825 is capable of hyperproducing dextranase at low pH to provide biologically contaminant-free supernatant liquid containing dextranase. Lipomyces starkeyi (IGC 4047), when grown on dextran as a sole carbon source produced a dextranase able to hydrolyse blue dextran and Sephadex G-100. The molecular weight was 23kDa and the isoelectric point was 5.4 [97]. The dextranase of L. starkeyi (ATCC 20825) studied by Koening and Day (1988, 1989a, 1989b) was analysed by SDS-PAGE and produced four bands, of molecular weights 65 kDa, 68 kDa, 71 kDa, and 78 kDa. Millson and Evans (2007) have isolated extracellular dextranase of L. starkeyi NCYC 1436 and have found that for their strain the enzyme occurs as three molecular weight species and seven isoelectric forms [68]. L. starkeyi nutrients (YPDex / YPD) The main ingredient in the chosen media is yeast extract. Yeast extract is a dried autolysate which facilitates rapid and luxuriant growth when used in various media or fermentation broth. It is a good source of amino-nitrogen and vitamins, especially the water-soluble B-complex vitamins. However, yeast extract is reported to enhance glucose metabolism to lipids, but inhibit lipolysis [18]. The metabolic pathway consists of converting glycerol into pyruvate or glucose and then hydrolysis by a phosphatase gives glycerol again. The disruption of this metabolic pathway, could account for the seemingly truncated numerous bands that SDS gives after prolonged storage of the yeast. Mycological peptone is incorporated in the media and discourages bacterial growth because of its acidity. Environment that dextranases favour Dextranase activity is affected by temperature, pH, metal ions and nutrients. According to Lin Chen et al (2007), dextranase activity is optimized between temperatures of 10oC and 60oC at pH of 6.0 [12]. In the particular study, the effect of pH on enzyme activity was determined by varying the pH between 3.5 and 8.5 under the temperature of 30oC. The pH of 3.4-4.5, 5.0-7.5, and 8.0-8.5 were maintained by sodium acetate buffer (20mM), citrate and phosphate buffer (20mM) and sodium phosphate buffer (20mM) respectively. The effects of metal ions (AlCl3, CaCl 2, CoCl2, CuSO4, FeCl3, KCl, MgCl2, NaCl, NiSO4, MnCl2 and ZnCl2) and SDS on dextranase activity were assayed by incubation of dextranase with 1mM metal ions or 1 mM SDS at pH 4.5 for 3h at 37oC, and then the enzyme activity of dextranase was determined. Ravi Kiran Purama and Arun Goyal (2008) in a study for optimization of nutritional factors, estimated dextransucrase activity in the cell free extract of Leuconostoc mesenteroides. They analysed the regression coefficients and t-values of six ingredients: yeast extract, sucrose, intercept, K2HPO4, beef extract, peptone and Tween 80. Yeast extract, sucrose, beef extract, and K2HPO4 displayed a positive effect for enzyme production whereas, peptone and Tween 80 had a negative effect on enzyme production. The variables with confidence levels greater than 90% were considered as significant. Sucrose was significant at 99.99% confidence levels for dextransucrase production. K2HPO4 and yeast extract were found significant about 94% level for dextransucrase production. Beef extract was significant 91% for dextransucrase production. Peptone and Tween 80 were found insignificant with negative coeffficients for enzyme activities. Methods used for enzyme activity measurement Enzymatic activity is measured with the help of laboratory methods called enzyme assays. All enzyme assays measure either the consumption or production of product over time. Enzyme assays can be split into two groups according to their sampling method: continuous assays, where the assay gives a continuous reading of activity, and discontinuous assays, where samples are taken, the reaction stopped and then the concentration of substrates/products determined [11], [20]. Continuous assays: Spectrophotometry in which you follow the course of the reaction by measuring a change in how much light the assay solution absorbs Fluorimetric assay in which we make use of the difference in the fluorescence of substrate from product to measure enzyme reaction. These assays are in general much more sensitive than spectrophotometric assays, but can suffer from interference caused by impurities and the instability of many fluorescent compounds when exposed to light Calorimetric assay in which the heat released or absorbed by chemical reactions is measured Chemiluminescence in which the light emitted by some enzyme reactions is measured so as to detect product formation. The detection of horseradish peroxidase by ECL is a common method of detecting antibodies in western blotting Discontinuous assays: Radiometry in which the incorporation of radioactivity in substrates is measured Chromatographic assays measuring product formation by separating the reaction mixture into its components. This is usually done by high-performance liquid chromatography (HPLC), but thin layer chromatography can also be used. Although this approach needs a lot of consumables its sensitivity can be increased by labelling the substrates/products with a radioactive or fluorescent tag Methods and assays for dextranase activity measurement The large variability of available substrates makes it difficult to estimate the enzyme activity, because the reaction product is often an undefined mixture of sugar polymers. The existing assays try to compromise convenience, speed and accuracy [44] Viscosimetric analysis was among the first to be used [31], [35], [36]. This method measured the amount of enzyme which reduced the specific viscosity of the dextran solution by half in 10min. and it is more suitable when dextranase hydrolyses the dextran molecule at random, producing long oligosaccharides. Reducing-sugar assay or saccharogenic methods measure the rate of increase in reducing sugar as measured with the Somogyi assay, the 3,5-dinitrosalicylicacid method (DNS) [102], thiourea borax-modified O-toluidine colour reagent (35) and alkaline potassium ferricyanide solution (225). These methods test the presence of free carbonyl group (C=O). It is a simple method commonly used to analyze for reducing sugars produced from enzymatic hydrolysis of substrates such as starch and sucrose [67].The most common substrates applied are Dextran T2000,47 T-260,3 and T110 [54], [72]. A number of substances have been reported as interfering with DNS colour development and citrate is one of them. Acetate and citrate are reported to enhance colour development and the true antagonist in this reaction is the proton (H+) [96]. This method is based on the release of short coloured products from polymeric blue dextran and their selective colorimetric detection at 610-650nm after precipitation of the p olymer. DNS colorimetric assays reported in literature are often modifications of the method of Webb and Spender-Martins (1983). E. F. Khalikova and N. G. Usanov (2001) developed a dextranase assay using an isoluble substrate, namely, Sephadex G-200 with Remazol Brilliant Blue dye [45]. The action pattern of dextranase was then, studied by means of exclusion chromatography. Overall, this assay was reported as convenient for quantitative dextranase detection, relatively independent of the enzyme source, and is proposed as an inexpensive alternative to the known procedures utilizing coloured substrates. The dextranase substrates can be either dye-releasing or fluorogenic. The assay procedures based on these substrates are accurate, fast and can be recommended for dextranase-producing microbial screening and enzyme purification. Other assay procedures worth mentioning include a spectrophotometric method with the use of Blue Dextran developed by Kauko K. Makinen and Illika K. Paunio (2004) who recommend it for column chromatography [62], and a method based on simple titration, developed by Eggleston and Gillian (2005) for easy use at the sugar cane factory [19]. Fluorometric assays are based on measuring the fluorescence of the samples and the results are often compared to a series of standards of Penicillium sp. A very sensitive fluorometric assay using amino-dextran-70 coupled with fluorescent dye BODIPY (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester) as the substrate was described by M. Zhou et al. (1998). The BODIPY FL dye-labelled dextranase substrate is an amine-containing dextran derivative that is labelled with the pH-insensitive, green fluorescent BODIPY FL dye, resulting in almost total quenching of the conjugates fluorescence. The increase of the fluorescent degradation products of BODIPY FL dextran is proportional to the amount of dextranase activity [102]. A suspension of Sephadex in a buffer is supplemented with agar, sterilized, and poured in Petri dishes, and after the wells are filled with the test solution, they are left to incubate. The dextranase activity can be evaluated by the extent of halos around the holes due to the opalescence of Sephadex. Milson and Evans (2007), measured dextranase activity using SDS PAGE as described by Laemmli (1970), using both mini-gel and Protean II electrophoresis systems, and stained using Coomassie Blue [68], [56]. Molecular weight markers were used to construct a calibration curve, from which molecular weights of dextranase were determined. Native gel electrophoresis was performed, but the loading buffer and the gel lacked SDS and ÃŽÂ ²-mercaptoethanol and the samples were not heated prior to loading on the gel. In the same study, dextranase activity was estimated in SDS gels, without extraction, by a plate modified from the method of Lawman and Bleiweis (1991) [57]. FL versus DNS assay method The classic method (DNS) for measuring glycosidases through release of reducing activity is simple and inexpensive and, as cited above, has been modified in several studies so as to suit the researchers needs. It may, however, have some pitfalls. The reaction taking place is the following: aldheyde group oxidation> carboxyl group 3,5-dinitrisalicylic acid reduction-> 3-amino,5-nitrosalycilic acid (Nam Sun Wang, University of Maryland) The above reaction scheme shows that 1 mole of sugar reacts with 1 mole of 3,5-dinitrisalicylic acid. However, it is suspected that there are many side reactions, and the actual stoichiometry is more complicated than that previously described. Different reduced sugars yield different colour intensities; thus it is necessary to calibrate for each sugar. Apart from the oxidation, other side reactions may compete for the availability of 3,5-dinitrisalicylic acid. Consequently, the calibration curve may be affected and the intensity of the developed colour may be enhanced. Therefore, the method has low specificity and one must run blanks diligently if the colorimetric results are to be interpreted correctly and accurately [96]. Another obstacle to be dealt with when using DNS is non-linearity. One cause of non-linearity could be the common practice of diluting reaction products before quantification of reducing compounds and another is the insufficiency of substrates. The fluorometric assay (FL), seems to gain ground in the most recent studies as faster and more accurate and it seems to leave space for modifications and combined use with other methods (see  §1.3.1). A standard curve is constructed from Penicillium sp. and then compared with the one derived from Lipomyces starkeyi. As described in the previous paragraph dextranase activity is estimated by the increase of the fluorescent products of dextran degradation. However, if too many fluoro are conjugated to the dextran molecule undesired may come up. Molecular Probes TM seems to overcome this problem by removing as much of the free dye as possible and then assaying the fluorescent dextran by (TLC) to ensure that it is free of low molecular weight dyes. So, in general, FL seems to yield accurate curves. Millson and Evans (2007), used an assay of dextranase activity which was a variation on that reported by Zhou et al. (1998). In that study, fluorescence vs. dextranase activity produced a linear log [68], [102]. Purification of L. starkeyi dextranase Dialysis tubing Dialysis tubing is typically used for changing the buffering solution of a protein and is also a method for concentrating protein solutions by dialysis against a hygroscopic environment (e.g. PEG, Sephadex). The protein solution is contained within a membrane which permits solute exchange with a surrounding solution and whose pore size prevents the protein from escaping. Except for small volumes, this method is time-consuming [11]. Filtration Ultrafiltration Ultrafiltration (UF) is a variety of membrane filtration in which hydrostatic pressure forces a liquid against a semi-permeable membrane. Suspended solids and solutes of high molecular weight are retained, while low molecular weight solutes pass through the membrane. UF is not fundamentally different from microfiltration or nanofiltration, except in terms of the size of the molecules it retains. [11], [77]. SDS-PAGE Purification of Lipomyces starkeyi dextranase is carried out mainly by running a SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) analysis. The solution of proteins to be analyzed is first mixed with SDS, an anionic detergent which denatures secondary and non-disulfide-linked tertiary structures, and applies a negative charge to each protein in proportion to its mass. SDS binds in a ratio of approximately 1.4g SDS per 1.0g protein. The size of the protein is directly related to the distance it migrates through the gel. Dextranase molecules migrate as bands based on size. Each band can be detected using stains such as Coomassie blue dye [77]. Modifications to the polypeptide backbone, such as N- or O- linked glycolylisation, however have a significant impact on the apparent molecular weight. Thus, the apparent molecular weight is not a true reflection of the mass of the polypeptide chain. In most cases, SDS-polyacrylamide gel electrophoresis is carried out with a discontinuous buffer system in which the buffer in the reservoirs is of a different pH and ionic strength from the buffer used to cast the gel. After migrating through a stacking gel of high porosity the SDS-polypeptide complexes are deposited in a very thin zone (or stack) on the surface of the resolving gel. The discontinuous buffer system that is most widely used was originally devised by Orstein (1964) and Dvis (1964) [77]. The sample and the stacking gel contain Tris Cl (pH 6.8), the upper and lower buffer reservoirs contain Tris-glycine (pH 8.3) and the resolving gel contains Tris Cl (pH 8.8). All components of the system contain 0.1% SDS [56]. Precipitation methods of proteins Precipitation is widely used in downstream processing of biological products, especially proteins. It serves to concentrate and fractionate the target product from various contaminants, as in biotechnology industry where precipitation helps to eliminate contaminants commonly contained in blood. The underlying mechanism of precipitation is to alter the solvation potential of the solvent and thus lower the solubility of the solute by addition of a reagent. Precipitation is usually induced by any of the following methods [11]: Salting out Isoelectric point precipitation Precipitation with organic solvents Non-ionic hydrophilic polymers Flocculation by polyelectrolytes Polyvalent metallic ions Salting out This the most common type of precipitation. Normally a neutral salt is added, such as ammonium sulphate, which compresses the solvation layer and increases protein protein interactions. As the salt concentration of a solution is increased, more of the bulk water is associated with the ions. Consequently, less water is available to partake in the solvation layer around the protein, which exposes hydrophobic interactions, aggregate and precipitate from solution. Isoelectric point precipitation The isoelectric point (pI) is the pH of a solution at which the net primary charge of a protein becomes zero. At a solution pH that is above the pÃŽâ„ ¢ the surface of the protein is primarily negatively charged and therefore like-charged molecules will exhibit repulsive forces. At a solution pH that is below the pI, the surface of the protein is primarily positively charged and repulsion between proteins occurs. At the pI, the negative and positive charges cancel, repulsive electrostatic forces are reduced and the dispersive forces predominate, and will, therefore, cause aggregation and precipitation. The pI of most proteins lies in the pH range of 4-6. Mineral acids, such as hydrochloric and sulphuric acid are used as precipitants. The greatest disadvantage to isoelectric point precipitation is the irreversible denaturation caused by the mineral acids. For this reason isoelectric point precipitation is most often used to precipitate contaminant proteins, rather than target pro tein. Precipitation with organic solvents Ethanol or methanol, if added to a solution may cause the proteins of the solution to precipitate. As the organic solvent gradually displaces water from the surface of the protein and binds it in layers around the organic solvent molecules, the solvation layer around the protein decreases. In that state, the protein can aggregate by attractive electrostatic and dipole forces. Parameters to consider are temperature (should be less than 0 °C to avoid denaturation), pH and protein concentration of the solution. Miscible organic solvents decrease the dielectric constant of water, which in effect allows two proteins to come together. At the pI the relationship between the dielectric constant and protein solubility is given by: log S = k/e2 + log S0 S0 is an extrapolated value of S, e is the dielectric constant of the mixture and k is a constant that relates to the dielectric constant of water [98]. Non- ionic hydrophilic polymers Dextrans, polyethylene glycols and other polymers are used in precipitation of proteins due to their low flammability and are less likely to denature biomaterials compared to pI precipitation. These polymers attract water molecules away from the salvation layer around the protein, which enforces protein-protein interactions and induces precipitation. For the case of polyethylene glycol, the following equation models precipitation: ln(S) +pS = X ÃŽÂ ±C C is the polymer concentration, P is a protein-protein interaction coefficient, ÃŽÂ ± is protein- polymer interaction coefficient and X = ( ÃŽÂ ¼i ÃŽÂ ¼i0 )RT ÃŽÂ ¼ is the chemical potential of component I, R is the universal gas constant and T is the absolute temperature [98]. Flocculation by polyelectrolytes Polyelectrolytes form extended networks between protein molecules in solution. These include alginate, carboxylmethylcellulose, polyacrylic acid, tannic acid and polyphosphates. The pH of the solution determines the effectiveness of these polyelectrolytes. Anionic polyelectrolytes are used at pH above the pI. Cationic polyelectrolytes are used at pH above the pI. The precipitate may dissolve back into the solution if an excess of polyelectrolytes is used. Polyvalent metallic ions Enzymes and nucleic acids are precipitated with the use of metal salts at low concentrations. Most frequently polyvalent metallic ions used are Ca+, Mg+, Mn+ or Fe+. Precipitation reactors Industrial scaled reactors that are used to precipitate large amounts of proteins, such as recombinant DNA polymerases from a solution include: Batch reactors The agent is slowly added to the protein solution under mixing, so the aggregating particles tend to be regular in shape. The protein particles are exposed to a wide range of shear stresses for a log period of time and become mechanically stable. Tubular reactors The precipitating reagent and the feed protein solution are contacted in an area of mixing and then added into enlongeted tubes where precipitation occurs. Plug flow is approached by the elements as they move along the tubes. The tubular reactor is inexpensive to be constructed but can become long and slow in case that aggregation of the particles occur slowly. Continuous stirred tank reactors CSTR reactors also known as vat or back mix reactors, run at steady state with a continuous flow of reactants and products in a well-mixed tank. It is a type of reactor mainly used in chemical engineering. A CSTR often refers to a mathematical model which is used to estimate the key unit operation variables when using a continuous agitated-tank reactor to reach a specified output. Perfect mixing is demanded. Precipitation of L. starkeyi The most common precipitation methods in the case of L. starkeyi cited in literature are: Isoelectric focusing Koening and Day (1988) used precast IsoGel agarose isoelectric focusing plates, pH 5.0-8.5. A standard mixture of proteins was applied in the lane next to each sample and the protein profile was quantified by densitometer scans. The enzyme activity in the gel was determined by slicing an unstained gel into 0.9 mm sections. Each section was placrd in a test tube with 1.0 ml 0.05 M citrate/phosphate (pH 5.5) buffer, allowed to elute overnight at 4oC and assayed for enzyme activity. This method separated the protein mixture into five isoelectric bands. All five forms were found to have dextranase activity and exhibited the same Km values. Organic solvents Polyethylene glycol precipitation is often used. Nishimura et al. (2002) used this method in an effort to prepare total DNA from L. starkeyi for taxonomy analysis. They added phenol solution (phenol: chloroform: isoamyl alcohol=25:24:1) to a test tube of Tris-SDS. The

Washington :: essays research papers

ASHINGTON, Jan. 6 — The Pentagon is pushing for a substantial increase, in the range of $20 billion or more, for its 2003 budget, confident that the war on terrorism has strengthened Congressional and public support for rebuilding the armed services, senior military officials say. Even as Congress is projecting a budget deficit next year, the Pentagon is arguing that it will need significantly more money to cover rising health care costs, stockpile precision-guided munitions and accelerate an array of big-ticket programs, including fighter jets and warships. "There is a real necessity to do something we didn't really address in the last budget, which is ramp up procurement," said Under Secretary of Defense Dov S. Zakheim, the Pentagon comptroller. "It's got to go up." Secretary of Defense Donald H. Rumsfeld has also vowed to use the budget for the 2003 fiscal year, which begins Oct. 1, to advance programs he has said will "transform" the military, including missile defense, unpiloted aircraft and high-tech battlefield communications equipment. Mr. Rumsfeld is expected to argue, for instance, that the armed services must stockpile laser- and satellite- guided weapons for future conflicts. Those munitions, which cost from $20,000 to $1 million each, exceeded expectations in Afghanistan, where they were used so extensively that the Navy arsenal came close to running dry, officials say. The secretary will also call for developing munitions that can penetrate caves and hardened, deeply buried bunkers — one area where military officials say American bombs were not always effective in Afghanistan. North Korea and Iraq are thought to have built many such bunkers for command centers and storage sites for biological and chemical weapons. Officials say Mr. Rumsfeld will also push for accelerating a program to convert Trident submarines, which are now armed with nuclear- tipped missiles, so they can instead carry precision-guided cruise missiles with conventional warheads. Dr. Zakheim said the Pentagon budget was still being negotiated with the White House and declined to provide figures. But senior military and Congressional officials have said the increase will be about $20 billion over the current $329 billion Pentagon budget, or about 6 percent, after adjusting for inflation. A $20 billion increase in the Pentagon budget would be less than the $33 billion increase approved by Congress last year, the largest since the Reagan era. But the new request comes in an economic downturn, when other federal agencies are being told to trim spending to balance declining tax revenues.

British Perspective on the Boston Tea Party Essay

In 1773 parliament passed the tea act in which the British pay less for tax to ship places. This made the prices of tea lower from Britain. Since Boston’s tea would be more expensive nobody would buy it from them. The tea act was just another problem adding up between the colonists and britain. This made the colonists want to be independent from Britain. The colonists decided to rebel and dumb three hundred and forty two chests of tea into the Boston Harbor. The act was given the name the Boston Tea Party. Most of the British thought of the Boston Tea Party as an act of terrorism. Really the Boston Tea Party was just another step to independence for the colonists. In 1763 the British put a line along the Appalachian Mountains so the the colonists could not move into the Ohio River Valley. This was called the Proclamation Line. The British prohibited the colonists from their land to protect them from the Indians. The colonists who went against the British and crossed the line were later killed by the Indians and then asked for the British to protect them again. When the colonists asked for help they British were still in a lot of debt from the war they fought to protect the colonists. They put British soldiers on the line to protect the colonists. This was a little bit rude of the colonists to try moving into the Ohio River Valley when the British were trying to help them. The fact that the British were forbidding the colonists from passing the Appalachian Mountains made them very mad. They looked at it as the British not allowing them to settle into more land. The colonists were not allowed to buy and live on the land that they fought and won a war for. It was there land and they were being deprived their right to their own property. The Sugar Act happened in 1764, the next thing to make the colonists angry. The sugar act put tax on sugar from other countries making it more expensive. The sugar was now more expensive than the British sugar. The British perspective on it was logical because they were in major debt from  the war they fought for the colonists. They were just hoping that the colonists would by their tea so they could use the money to pay off the debt. They thought they were being fair by not taxing their sugar so the colonists would not have to pay a tax. The way the colonists saw the Sugar Act was an act of selfishness. The British taxed the tea the colonists used to buy because it was cheaper. The British had no right to tax the colonists. They had no say in how much the tax should be or what it was on. They used sugar everyday it was completely unfair. In 1765 the British made the Quartering Act. It required for british troops to have to live in the colonists homes when they were stationed in the colonies. The British thought it was only fair for the colonists to take care of their soldiers. Many of those soldeirs fought in the war against the French and Indians. It would cost too much for Britain to pay to build housing for the soldiers. The colonists did not like the Quartering act. They did not want to take care of the soldiers. First of all because the soldiers were the ones from keeping them to get to the rest of their land in the Ohio River Valley. Now they were being forced to take care of the soldiers. Nobody wanted to because they did not even have enough room in their homes. They did not have enough money to clothe and feed them either. The soldiers were very rude to the colonists and kind of scared most of them too. In 1767 a powerful British man named Charles Townshend had the idea of putting taxes on glass, lead, paints, paper, and other everyday products of the colonists. The reason for taxing these products was so Britain could get more money for their government. They also did it for more power over everyone. The colonists did not like the Townshend acts. It was just a way for Britain to take more money. The colonist rebelled just like they did with the quartering and sugar act. They boycotted British products. The trade between  them dropped by fifty percent as a result in the boycott. They claimed it was unconstitutional because the colonies did not have any say in the Parliament. The debate wether or not the colonies had represntation in parliament was not settled until the revolution. The Tea Act was passed by the British Parliament in 1773. The British made it so the colonists could not but any tea from any one else besides the East India Company. The East India Company was poor and the British wanted to help them with their business. The tea act made the price of tea lower from the East India Company so everyone would buy from them. They said that it was a benefit to the colonists because they could get tea cheaper now. The tax on the tea was very minimal and they just wanted to put a tax on it to show they have the right to tax. This made the Colonists look at the Tea Act as another taxation without representation situation. It meant they could not buy tea from anyone else and so their tea would lose its business. They were also angry because it went against the english right of only being taxed by the elected representatives not the parliament. The Sons of Liberty were a group of patriots who were dedicated to getting independence for the colonies. They Sons of Liberty Believed in being free. They were a secret group that wanted to fight for America. The group formed when the British Empire became on of the most feared powers in the world. Samuel Adams formed the Sons of Liberty. The British saw the Boston Tea Party as an act of terrorism. They saw it as a group of drunk and crazy people dumping their tea into the boston Harbor with out paying for it. They completely rebelled against the government and wasted a lot of money after all that the British have done for them. On December 16, 1773 the Sons of Liberty boarded three British ships. They dressed up as Mohawk Indians in order to be able to access the ships. They also had to arm themselves with hatches and axes to make their disguises more believable. They opened and dumped every single chest into the Boston  Harbor. This was called the Boston Tea Party. When it was over there were three hundred and forty two wooden chests floating in the water. Many other people boycotted the tea in the same way later on. The colonists did this to show independence. I can see how the British thought the Boston Tea Party was an act of Terrorism. I believe that the Boston Tea Party was not an act of terrorism. Britain took advantage of the colonists. Even though they tried helping the colonists a few times they still should not have used us to make more money for their government. They should be able to run and take care of their own money issues without taking the colonists money. The colonists were definitely right in taking action and rebelling against the British in every way they could. The Boston Tea Party was just another step closer to the independence of America.

American Spanish war essays

American Spanish war essays The event and country under scrutiny is the United States of America and the 1898 Spanish American War. The concepts relevant to the Event and country chosen are Militarism, Imperialism and traditional diplomacy/war. To explain, Militarism is the desire to use the armed forces of a country in order to make that country more powerful. Imperialism is a system of rule in which a rich and powerful country controls other countries in order to become richer and more powerful and Traditional Diplomacy and/ war is the management of relations between countries or a period of fighting between countries or states. In reference with the concepts Diplomacy and War the immediate origins of the 1898 Spanish American War began with the Wilson-Gorman Tariff of 1894. The American tariff, put restrictions on sugar imports to the United States, severely hurting the economy of Cuba, which was based on production and the sale of sugar. In Cuba, then a Spanish colony, disappointed nationalists known as Insurrectos (Cuban Nationalists who fought against Spain's Colonial regime in Cuba), began to revolt against the ruling Spanish Colonial regime. By the time President McKinley, a diplomat came into office in 1897, the uproar over Cuba was continuing. In 1898, the US dispatched the USS Maine on a friendly mission to Cuba. The ship was to wait, ready to rescue US citizens who might have been endangered by the conflict in Cuba. On February 15, 1898, the Maine mysteriously blew up, causing American feelings toward Spain to turn hostile. Immediately the blame was directed to a Spanish mine. And it was then that McKinley declared war between Spain and America and by April, 1898 war had begun. In order to assure the world that it was fighting only for the good of Cuba and not for colonial gain, the United States passed the Teller Amendment, which provided the US with justification for its actions while allayin ...

Present tense beach onbservation essay

Present tense beach onbservation essay The BeachMy favorite place to be is the eveneing beach. The activities and beautiful sightsintrigue me. It can also make me miss being a child, because I see how excited the littlegirl in her pink bathing suit gets so excited with a fairly large hole already dug in the sand.Many people feel that there is very little activity at the beach and it is just a laid backplace. I beg to differ, if you gaze out way into the sky a rainbow like dot sits as a mandangles beneath it amazed at the sight below him. Suddenly, there is a strange dongbehind me comming from the hotel, but no one cares because overriding the sound is ahuge yellow speedboat with the word thriller wrote in red on the side. The kids at theshore hurry to the water anxiously waiting for the man made waves to appear.SwimsuitAs I look atthe water it seems like an ocean full of diamonds. The sand almost has the same affectonly instead of diamonds it is a ground drenched in glitter because of the beams ofsunlight reflecting off o f every grain of sand. The best part of the beach is the sun andthe clouds. The sun painting so many splashes of colors as the clouds dance around itforming shapes as your imagination defines them.

The Culture of Zulu Research Paper Example | Topics and Well Written Essays - 2000 words

The Culture of Zulu - Research Paper Example Zulus placed a lot of importance on their ancestors and their spirits. These ancestors were supposed by them to be living in Unkulunkulu which was considered as the world of the spirits. The ancestors were the link between the living and the spirits. For this reasons they needed to be remembered by the family members, praised regularly and also appeased by making offerings to them. In case a family failed to do so, the spirits are said to have visited them in the form of troubles. These troubles can be solved only if the mistake of the family is rectified and the spirits of the ancestors are pacified by some offerings and by showering of praises on them. The Sangoma was the spiritual healer and the priest of the Zulus. In the case of a mishap such as failure of crops, the death of livestock, drought or any other natural calamity the Sangoma was the one who declared the reasons for the calamity. The calamity could have been caused by the unhappy spirits or by witchcraft. If it was the former, the family was supposed to appease the ancestors but if it was the latter a witch-hunt and the elimination of the witch responsible was carried out. Another important religious person among the Zulus is the Inyanga. The Inyanga can be said to be the doctor of the Zulu tribe. He mostly used local plants and animals in order to find a cure of the common diseases like cuts, bruises. The cure was usually followed by religious rituals in which the spirits were praised and pacified and asked to restore the health of the person who is sick.

New Product Packaging Research Paper Example | Topics and Well Written Essays - 1000 words

New Product Packaging - Research Paper Example It is important to note that the consumers recognize the colors and other forms that trigger the visual stimuli in the cognitive mind (Johnson, 2012). Once this happens the as, a survival instinct works, and you engage the consumer by the attraction nature. We shall use pink, blue and print the letters in black for total engagement. Pink is a color that symbolizes nurturing and unconditional love, giving the car owner the sense of love to the car and matching it with the product. Blue symbolizes the brand loyalty and confidence in the product; this communicates the sense of friendliness and consumer importance to us. The black letters print will represent the strength and brand authority also makes it stand out from the others, the letters are printed in aerial fonts for easier reading.The cartoon must have a simple design on one side indicating the number of piles for piling together plus other information like temperature and keeping it up. On the opposite side, it contains the add ress and location of the company. The other two sides show the content inside the carton. The simple design on the carton is effective in communication and branding. The print on the carton is tailor made to connect with the product inside hence the use of graphic design on two sides.For the small runs, flexography printing methods will be suitable for our production. It allows printing of up to three colors this make it a viable option for a high-quality small runs tailor made cartons for packaging the car perfumes.